|
KMID : 0613820180280121529
|
|
Journal of Life Science
2018 Volume.28 No. 12 p.1529 ~ p.1535
|
|
|
Isolation and Identification of Ampicillin-resistant Bacteria in Changwon
|
|
Bae Young-Min
|
|
|
Abstract
|
|
|
|
The number of antibiotic-resistant bacteria is increasing rapidly while the discovery rate of new antibiotics is in decline. A systematic study is therefore necessary to investigate which bacteria are resistant to medically important antibiotics and how high that resistance is. To that end, this study aimed to analyze which bacteria demonstrated resistance to ampicillin, one of the currently most- widely used medical antibiotics. Water samples were collected from the Changwon-Cheon that runs through Changwon City and from the pond in front of the dormitory building at Changwon University. Hundreds of ampicillin-resistant colonies were obtained and 22 morphologically distinct examples were chosen for further study. These bacteria were identified by amplifying their 16S rRNA genes and comparing those sequences with data in GenBank. The bacteria was identified as belonging to 10 families, 12 genera, and 17 species, and all were able to grow in the presence of 50 μg/ml ampicillin while seven showed growth at ampicillin concentrations as high as 1.5 mg/ml.
|
|
|
KEYWORD
|
|
|
Ampicillin, antibiotic resistance, polymerase chain reaction, 16S rRNA gene
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|